ADVANCED PHASE CONTRAST MICROSCOPE KCX-199

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• 1. High-Contrast Infinity Corrected Optical System
• The KCX-199 Advanced Phase Contrast Microscope features a high-contrast infinity-corrected optical system designed to provide clear and detailed images of microscopic specimens.
• This optical configuration supports biological specimen observation and integration with suitable advanced microscopy accessories.
• 2. Siedentopf Trinocular Viewing Head
• The microscope incorporates a Siedentopf trinocular observation head inclined at 30° with full 360° rotation.
• Its interpupillary adjustment range of 48–75 mm and diopter adjustment allow users to customize the viewing position.
• 3. WF10X / 22 mm Wide Field Eyepieces
• The microscope features WF10X wide-field eyepieces with a 22 mm field number.
• Antifungal and anti-reflection coatings are specified to support optical clarity and routine laboratory use.
• 4. Semi-Plan Infinity Objective Lenses
• The KCX-199 includes semi-plan infinity objective lenses designed for clear specimen observation and improved field flatness.
• The supplied objective configuration includes 4X, 10X, 40X spring-loaded and 100X oil immersion spring-loaded lenses.
• 5. 40X–1000X Magnification Range
• The microscope provides optical magnification from 40X to 1000X, allowing users to examine specimens at different magnification levels.
• This range supports routine biological observation, laboratory education and scientific investigations.
• 6. Inward Quintuple Revolving Nosepiece
• The microscope features an inward-facing quintuple revolving nosepiece with click stops and rubber grip.
• This mechanism allows convenient switching between objective lenses and provides an additional objective position.
• 7. Large Rackless Mechanical Stage
• The 190 × 150 mm mechanical stage features a rackless, scratch-resistant design with an X/Y movement range of 75 × 50 mm.
• Double specimen holder clips support secure positioning of prepared microscope slides.
• 8. Coaxial Coarse and Fine Focusing
• The KCX-199 incorporates coaxial coarse and fine focusing controls for convenient image adjustment.
• The supplied focusing specification includes 0.2 mm per rotation, supporting controlled movement during specimen examination.
• 9. Movable Abbe Condenser N.A. 1.25
• The microscope features a movable Abbe condenser with numerical aperture 1.25, an aspheric optical element and antifungal and anti-reflection coatings.
• The condenser helps concentrate and control illumination for transmitted-light observation.
• 10. Adjustable 3.5W LED Illumination
• The microscope incorporates a 3.5W LED illumination system with adjustable intensity control.
• The LED is specified with a rated working life of up to 100,000 hours, depending on operating conditions and component specifications.
• 11. Heavy-Duty Aluminum Die-Cast Stand
• The KCX-199 features a single-mould aluminum die-cast stand designed for stability and corrosion resistance.
• Its sturdy construction supports regular laboratory operation and educational use.
• 12. Universal Power Supply
• The microscope supports 110–240V electrical input through its universal power supply.
• An integrated voltage fluctuation stabilizer is specified to support stable illumination operation.
• 13. Adjustable Rubber Eye Guards
• The microscope includes adjustable rubber eye guards designed to improve viewing comfort during laboratory observation.
• 14. Trinocular Camera Compatibility
• The trinocular viewing head provides an optical path that may be used with compatible microscope cameras and appropriate adapters for digital image capture and documentation.
• 15. Multiple Laboratory Applications
• The microscope is suitable for biological observation, scientific research, laboratory training, educational demonstrations and routine examination of prepared specimens.

Biological Laboratory Observation

The KCX-199 Advanced Phase Contrast Microscope is designed for examining biological specimens and prepared laboratory slides.

Its infinity-corrected optics and multiple objective lenses support observation of suitable cells, tissues and microscopic structures.

Scientific Research Laboratories

Research laboratories can use the KCX-199 for routine biological microscopy and laboratory investigations.

Its mechanical stage, precision focusing controls and adjustable illumination provide a practical configuration for specimen examination.

Educational Institutions and Universities

Schools, colleges, universities and scientific training centers can use the microscope for biological practical sessions, laboratory demonstrations and microscopy education.

Cell Biology Research

The microscope can support examination of suitable cell specimens and prepared slides.

Phase-contrast observation of transparent, unstained cells requires compatible phase objectives and a matching phase condenser or phase annulus system.

Microbiological Observation

The KCX-199 can be used for observing appropriately prepared and stained microbial specimens.

Its 40X and 100X objectives support high-magnification examination within the capabilities of conventional optical microscopy.

Pathology and Laboratory Training

The microscope can support educational observation of prepared tissue sections and pathological teaching slides.

Any use for clinical diagnosis requires appropriate validation and compliance with applicable requirements.

Digital Microscope Imaging

The trinocular observation head can support integration with compatible digital cameras through suitable adapters.

This allows microscope images to be captured or displayed digitally when the required imaging accessories are installed.

Pharmaceutical Research Laboratories

Pharmaceutical research and educational laboratories can use the microscope for suitable specimen observation, laboratory training and routine visual examination.

Scientific Demonstrations

The microscope can be used for classroom teaching, laboratory workshops and technical training involving optical magnification, specimen focusing and biological observation.

Working Principle

The Advanced Phase Contrast Microscope KCX-199 operates using compound optical magnification through an infinity-corrected optical system.

A prepared specimen is placed on the mechanical stage and positioned within the optical path.

Light from the adjustable 3.5W LED illumination system passes through the condenser and specimen before entering the selected objective lens.

The semi-plan infinity objective forms an optical image that is processed through the microscope's infinity-corrected optical system and viewed through the WF10X eyepieces.

The coaxial coarse and fine focusing controls allow users to adjust image sharpness, while the mechanical stage enables controlled specimen movement.

Phase Contrast Working Principle

Phase-contrast microscopy converts phase differences caused by variations in specimen thickness and refractive index into visible differences in image brightness.

In a correctly configured phase-contrast system, an annular diaphragm in the condenser produces a hollow cone of illumination. The objective's phase plate modifies the phase relationship between direct and specimen-diffracted light.

Interference between these light components produces contrast, allowing suitable transparent specimens to be observed without conventional staining.

Important: The supplied KCX-199 specifications list an Abbe condenser and semi-plan objectives but do not explicitly identify a phase annulus condenser or phase-contrast objectives. These components must be confirmed before claiming that the supplied configuration performs phase-contrast observation.

Optical Magnification Combinations

Microscope Compatibility and Usage

The KCX-199 Advanced Phase Contrast Microscope is suitable for observation of compatible prepared biological slides and laboratory specimens.

It can be used in universities, research laboratories, biological laboratories, scientific training centers and educational institutions.

The trinocular observation head can support compatible digital imaging accessories, subject to the appropriate camera adapter and optical configuration.

For routine observation, users should select the appropriate objective lens, adjust LED illumination and use the coarse and fine focusing controls to obtain a clear specimen image.

The 100X oil immersion objective should be used with suitable immersion oil according to the microscope's operating instructions.

Phase-contrast operation requires confirmation of the appropriate phase objectives and matching condenser components.