FLUORESCENT MICROSCOPE KCX-162

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• 1. Advanced Research Fluorescence Microscope
• The KCX-162 Fluorescent Microscope is designed for advanced biological and fluorescence-based research applications.
• It supports magnified observation of suitable fluorescently labelled cells, tissues and microorganisms.
• 2. Infinity-Corrected Optical System
• The microscope features an infinity-corrected optical system.
• This configuration is designed to provide clear optical imaging and support compatible intermediate optical accessories within the microscope's optical path.
• 3. Plan-Achromatic Objectives
• The KCX-162 incorporates plan-achromatic objectives designed to improve field flatness and reduce chromatic aberration.
• These objectives support detailed examination of suitable biological specimens.
• 4. High-Magnification Optical Observation
• The supplied objective range extends from 4X to 100X.
• With the specified WF10X eyepieces, these objective magnifications correspond to nominal optical magnifications of 40X to 1000X.
• 5. Up to 2500X Specified Magnification
• The supplied reference lists a maximum magnification of 2500X with an appropriate optical configuration.
• This figure depends on the selected eyepiece, objective and any additional optical magnification components.
• 6. High-Resolution Oil Immersion Objective
• The microscope includes a 100X oil immersion objective with N.A. 1.25.
• This objective supports high-magnification observation when used with appropriate immersion oil and specimen preparation.
• 7. Trinocular Viewing Head
• The KCX-162 features a trinocular observation head designed for binocular viewing and compatible camera integration.
• 8. Comfortable 30° Inclined Head
• The microscope incorporates a 30° inclined viewing head intended to provide a convenient observation position during laboratory work.
• 9. Adjustable Interpupillary Distance
• The microscope supports an interpupillary adjustment range of 48–75 mm.
• This allows users to adjust the distance between the eyepieces according to their viewing requirements.
• 10. High Wide-Field WF10X Eyepieces
• The KCX-162 features extra-wide-field WF10X eyepieces for direct optical specimen observation.
• 11. Inward-Facing Quadruple Nosepiece
• The microscope incorporates an inward-facing quadruple revolving nosepiece.
• This arrangement supports convenient selection of the installed objective lenses.
• 12. Multi-Channel Fluorescence System
• The microscope features a multi-channel fluorescence filter system supporting blue, green and ultraviolet excitation wavelength ranges.
• This provides flexibility for observing compatible fluorescent labels.
• 13. Blue Fluorescence Filter
• The reference specifies a blue excitation range of 420–485 nm.
• This range may be suitable for selected fluorescent labels when the complete filter set and illumination spectrum are compatible.
• 14. Green Fluorescence Filter
• The microscope lists a green excitation range of 480–550 nm.
• This range supports suitable fluorescence observation when paired with compatible emission filtering and fluorescent materials.
• 15. Ultraviolet Fluorescence Filter
• The KCX-162 includes a specified ultraviolet excitation range of 330–400 nm.
• This supports suitable UV-excited fluorescence applications with the appropriate optical filter configuration.
• 16. 100W HBO Mercury Fluorescence Lamp
• The microscope incorporates a 100W HBO high-pressure mercury lamp for fluorescence excitation.
• The lamp provides illumination that can be filtered for selected fluorescence observation applications.
• 17. Adjustable Fluorescence Illumination
• The supplied reference specifies adjustable fluorescence brightness.
• This allows illumination to be regulated according to the selected specimen and imaging requirements, subject to the installed lamp control system.
• 18. Bright-Field Observation Capability
• The KCX-162 supports conventional bright-field microscopy in addition to fluorescence observation.
• This allows users to examine suitable stained and prepared biological specimens using transmitted light.
• 19. Double-Layer Mechanical Stage
• The microscope incorporates a double-layer mechanical stage designed for controlled specimen positioning.
• 20. Smooth X-Y Specimen Movement
• The mechanical stage supports smooth movement along the X and Y axes.
• This allows different regions of a prepared specimen to be examined.
• 21. Rack-and-Pinion Condenser
• The microscope features a rack-and-pinion condenser for controlled positioning of the transmitted-light illumination system.
• 22. High Numerical Aperture Condenser
• The KCX-162 includes a condenser with N.A. 1.25.
• This supports appropriate illumination control for compatible transmitted-light microscopy configurations.
• 23. Adjustable Light-Path Alignment
• The condenser arrangement supports light-path alignment adjustments for optimizing specimen illumination.
• 24. Digital Camera Compatibility
• The trinocular observation head allows a compatible digital camera to be attached using suitable imaging adapters.
• 25. Research Documentation Support
• With appropriate camera and software accessories, the microscope can support digital image capture and research documentation.
• 26. Immunofluorescence Applications
• The KCX-162 supports observation of appropriately labelled specimens using compatible fluorescence filter sets.
• 27. Cell Biology Applications
• The microscope is suitable for observing prepared cellular specimens and selected fluorescent markers.
• 28. Microbiology Research
• The microscope can be used for fluorescence-based observation of appropriately prepared microbial specimens.
• 29. Biotechnology and Pharmaceutical Research
• The KCX-162 supports suitable laboratory research involving biological specimens, fluorescent labels and microscopy-based investigations.
• 30. Advanced Laboratory Education
• The microscope can be used for teaching fluorescence microscopy, optical magnification and biological specimen examination.

The Fluorescent Microscope KCX-162 is designed to support advanced microscopic examination of biological specimens using both fluorescence and conventional bright-field observation techniques.

Fluorescence microscopy is used when selected biological structures are labelled with fluorescent materials or exhibit suitable natural fluorescence.

The microscope illuminates the specimen with excitation light from its 100W HBO mercury lamp through an appropriate optical filter arrangement.

Fluorescent molecules within the specimen absorb excitation light and emit light at characteristic wavelengths.

The microscope collects the emitted light and directs it through the optical system for magnified observation.

The multi-channel fluorescence system allows users to select among blue, green and UV excitation configurations according to the fluorescent labels and filter sets available.

The microscope's plan-achromatic objectives support examination of suitable biological structures, while its trinocular head enables integration with compatible digital imaging equipment.

Conventional bright-field microscopy can also be used for examining prepared biological specimens without fluorescence illumination.

The KCX-162 is therefore intended for immunofluorescence research, cell biology, microbiology, pathology research, biotechnology and scientific education.

Applications

1. Immunofluorescence Research

The KCX-162 Fluorescent Microscope can be used to examine biological specimens labelled with compatible fluorescent antibodies.

Its multi-channel fluorescence configuration supports appropriate immunofluorescence observation workflows.

2. Cell Biology Research

Cell biology laboratories can use the microscope to examine suitable cellular structures, morphology and fluorescent markers.

3. Pathology Research

The microscope can support research-oriented examination of appropriately prepared tissue and cellular specimens.

4. Microbiology Laboratories

Microbiology researchers can use the KCX-162 for suitable fluorescence-based observation of labelled microorganisms.

5. Fluorescent Staining Studies

The microscope supports examination of specimens prepared using compatible fluorescent staining methods.

6. Cellular Morphology

The KCX-162 can be used to examine suitable cellular structures through bright-field and fluorescence microscopy.

7. Molecular Biology Research

The microscope can support observation of fluorescently labelled biological materials used in suitable molecular biology investigations.

8. Pharmaceutical Research

Pharmaceutical research laboratories can use the microscope for appropriate cellular and fluorescence-based investigations.

9. Biotechnology Laboratories

The microscope is suitable for research involving fluorescent markers, prepared biological specimens and compatible microscopy assays.

10. Medical Research Laboratories

The KCX-162 can support research-oriented observation of biological samples in medical research environments.

11. University Research Departments

Universities can use the microscope for advanced biological microscopy teaching and laboratory research.

12. Scientific Research Institutes

The microscope supports suitable fluorescence-based investigations in research institutions.

13. Biological Specimen Examination

The KCX-162 can be used to examine appropriately prepared tissues, cells and microorganisms.

14. Diagnostic Method Development

Research laboratories can use the microscope to investigate fluorescence-based laboratory methods.

Use for actual clinical diagnosis requires appropriate assay validation, regulatory compliance and approved operating procedures.

15. Fluorescence-Based Microbial Studies

The microscope may support laboratory research involving fluorescently labelled microbial specimens and compatible detection methods.

16. Digital Microscopy Documentation

The trinocular head can be integrated with compatible cameras for recording selected microscope images.

17. Advanced Scientific Education

The microscope is suitable for teaching fluorescence principles, optical imaging and laboratory microscopy techniques.

18. Specialized Laboratory Research

The KCX-162 supports appropriate research applications requiring multiple fluorescence excitation ranges and high-magnification observation.

Working Principle

The Fluorescent Microscope KCX-162 operates using the principles of optical magnification, fluorescence excitation, fluorescence emission and wavelength-selective optical filtering.

A suitably prepared biological specimen is placed on the microscope's mechanical stage.

The 100W HBO mercury lamp produces light containing multiple spectral components.

An excitation filter selects the wavelength range required to illuminate the fluorescent material within the specimen.

The selected excitation light is directed toward the specimen through the fluorescence illumination optics.

Fluorescent molecules absorb the excitation light and enter an excited electronic state.

As these molecules return to a lower energy state, they emit light, typically at a longer wavelength than the excitation light.

The microscope objective collects the emitted fluorescence.

The fluorescence filter arrangement separates the desired emitted light from unwanted excitation illumination.

The resulting optical image passes through the microscope's infinity-corrected optical system and is observed through the trinocular viewing head.

A compatible camera can also be used for digital image acquisition when suitable accessories are installed.

Fluorescence Observation Process

1. Specimen Preparation: A compatible biological specimen is prepared using suitable fluorescent labels or naturally fluorescent material.

2. Excitation Light Generation: The 100W HBO mercury lamp produces illumination.

3. Excitation Filtering: The selected fluorescence filter isolates the required excitation wavelength range.

4. Specimen Excitation: Fluorescent molecules absorb the excitation light.

5. Fluorescence Emission: The fluorescent material emits light at characteristic wavelengths.

6. Emission Filtering: The optical filter arrangement separates desired fluorescence from unwanted excitation light.

7. Optical Magnification: The objective and microscope optical system form a magnified image.

8. Observation and Documentation: The image is viewed through the eyepieces or captured using a compatible camera.

Magnification System

Standard Optical Magnification Examples

10X and 40X objectives are illustrative intermediate magnifications within the stated objective range; their exact specifications should be confirmed for the KCX-162.

The 2500X specification requires a compatible additional optical configuration. Higher nominal magnification does not necessarily provide greater optical resolution.

Fluorescence Filter System

These wavelength ranges are taken from the supplied reference. They do not, by themselves, establish complete fluorescence filter-set compatibility.

The excitation filter, dichroic mirror, emission filter and fluorescent label must be matched for the intended application.

Digital Imaging Compatibility

Microscope Compatibility and Usage

The KCX-162 Fluorescent Microscope is intended for observation of suitable prepared biological specimens in scientific and research laboratories.

It can be used in universities, biotechnology laboratories, microbiology departments, pharmaceutical research centers and specialized scientific institutions.

For bright-field observation, the specimen is placed on the mechanical stage and the transmitted-light illumination system is selected.

For fluorescence observation, the operator must select a compatible filter configuration and use a specimen prepared for the relevant excitation and emission wavelengths.

The selected objective lens and illumination settings should be appropriate for the specimen.

The microscope's trinocular head may be integrated with compatible digital imaging accessories for documentation and research analysis.

The 100W HBO high-pressure mercury lamp must be operated only with its specified power supply, enclosed lamp housing, protective optical components and manufacturer-approved safety procedures. Users must not look directly at the lamp or unfiltered UV illumination. Lamp handling, replacement and disposal require appropriate trained personnel and procedures.

The microscope should be used according to its final technical configuration, laboratory safety instructions and applicable regulatory requirements.