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The Inverted Fluorescence Microscope KCX-166 by KC SCIENTIFIC WORKS features infinity-corrected optics, long working distance objectives, four-channel fluorescence, a 25MP CMOS camera and advanced imaging software. Designed for cell biology, immunofluorescence, biotechnology and biomedical research, it supports bright-field, phase-contrast, DIC and fluorescence observation with compatible digital imaging capabilit…
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| Parameter | Specification |
|---|---|
| Product Name | Inverted Fluorescence Microscope |
| Model Number | KCX-166 |
| Brand | KC SCIENTIFIC WORKS |
| Manufacturer | KC SCIENTIFIC WORKS |
| Microscope Type | Advanced Inverted Fluorescence Research Microscope |
| Optical System | Infinity-Corrected Optical System |
| Observation Modes | Bright Field, Phase Contrast, Fluorescence and DIC |
| Observation Head | Binocular Siedentopf Head |
| Head Inclination | 35° |
| Camera Port | Integrated Trinocular Camera Port |
| Eyepieces | WF10X / 22 mm High Eyepoint |
| Interpupillary Distance | 50–75 mm |
| Nosepiece | Six-Position Revolving Nosepiece |
| Objective Type | Long Working Distance Objectives |
| Objective Magnifications | 10X, 20X, 40X and 60X |
| Nominal Optical Magnification | 100X, 200X, 400X and 600X |
| Condenser | Universal Turret Condenser |
| Mechanical Stage | 250 × 160 mm Precision Mechanical XY Stage |
| Stage Travel | 114 × 73 mm |
| Focusing System | Coaxial Coarse and Fine Focusing |
| Transmitted Illumination | High-Intensity Scientific White LED |
| Fluorescence System | Four-Position LED Fluorescence Filter Turret |
| Fluorescence Filter Sets | DAPI, FITC, TRITC and CY5 |
| Camera Type | Scientific CMOS Camera |
| Specified Camera Resolution | 25 MP |
| Specified Image Resolution | 4800 × 3600 Pixels |
| Maximum Frame Rate | Up to 60 fps |
| Connectivity | USB 3.2 Gen 2, HDMI and Wi-Fi |
| Imaging Software | Imaging, Measurement, Time-Lapse, Z-Stack and AI Analysis |
| Additional Imaging Feature | Image Stitching, Subject to Software Configuration |
| Computer | Intel Core i7 Workstation |
| Computer RAM | 8 GB |
| Storage | 1 TB SSD |
| Operating System | Windows 10 / Windows 11 |
| Supplied Accessories | Dust Cover, Filters and Instruction Manual |
| Main Applications | Cell Biology, Live-Cell Imaging, Immunofluorescence, Biotechnology and Biomedical Research |
| Product Category | Inverted Fluorescence Research Microscopes |
The KCX-166 Inverted Fluorescence Microscope is designed for fluorescence-based biological research and observation of suitable specimens in compatible laboratory vessels.
Its inverted optical arrangement allows the objectives to view specimens from beneath the stage.
The microscope incorporates an infinity-corrected optical system designed to support high-quality imaging and compatible optical accessories.
This arrangement is useful in research microscopy systems requiring multiple observation techniques.
The KCX-166 supports the following observation configurations:
Bright Field
Phase Contrast
Fluorescence
Differential Interference Contrast (DIC)
Each mode provides a different method of visualizing suitable biological specimens.
The microscope positions its objective lenses beneath the specimen stage.
This arrangement supports observation of specimens in compatible culture dishes, laboratory plates and other suitable containers.
The microscope features a binocular Siedentopf viewing head designed for convenient optical observation.
The KCX-166 incorporates a 35° inclined observation head, providing a practical viewing angle for laboratory microscopy.
The microscope includes WF10X / 22 mm high-eyepoint eyepieces.
These eyepieces support wide-field optical observation.
The microscope supports an interpupillary adjustment range of 50–75 mm.
This allows users to adjust eyepiece spacing according to their viewing requirements.
The KCX-166 incorporates an integrated camera port for digital microscopy.
This allows compatible imaging equipment to receive the microscope image for display and documentation.
The microscope features a six-position revolving nosepiece.
This provides positions for the installed objectives and compatible additional objective configurations.
The KCX-166 incorporates long working distance objectives designed for inverted microscopy.
These objectives provide clearance for compatible specimen vessels and laboratory observation arrangements.
The supplied objective set includes:
10X Long Working Distance Objective
20X Long Working Distance Objective
40X Long Working Distance Objective
60X Long Working Distance Objective
These objectives support observation at different magnification levels.
The microscope features a universal turret condenser designed for compatible transmitted-light observation configurations.
The KCX-166 incorporates a high-intensity white LED illumination system for suitable transmitted-light microscopy applications.
The microscope features a four-position fluorescence filter turret.
This allows selection between the installed fluorescence filter configurations.
The KCX-166 includes a DAPI filter configuration for observing compatible DAPI-labelled specimens.
The microscope supports FITC fluorescence observation with an appropriately matched filter set and illumination system.
The KCX-166 includes a TRITC filter configuration for suitable fluorescently labelled specimens.
The microscope supports CY5 fluorescence imaging when the installed excitation and emission optics are compatible with the selected label.
The KCX-166 incorporates a 250 × 160 mm mechanical XY stage.
This supports stable positioning of compatible biological specimens and laboratory vessels.
The mechanical stage provides 114 × 73 mm X/Y travel.
This allows controlled specimen movement during microscopy.
The microscope features coaxial coarse and fine focusing controls.
These allow initial image adjustment and more precise focusing.
The KCX-166 includes a specified 25 MP scientific CMOS camera for digital specimen imaging.
The camera supports digital image acquisition and research documentation.
The supplied reference specifies an image resolution of 4800 × 3600 pixels.
This supports detailed digital specimen recording within the capabilities of the installed sensor and imaging system.
The digital imaging system lists a maximum frame rate of up to 60 frames per second.
The actual frame rate depends on image resolution, camera operating mode and connection bandwidth.
The camera system supports USB 3.2 Gen 2 connectivity for compatible high-speed data transfer.
The specified HDMI interface supports connection to compatible digital display equipment.
The imaging configuration includes Wi-Fi connectivity for supported wireless imaging workflows.
The KCX-166 includes imaging software designed for compatible image acquisition, observation and analysis functions.
The software supports image measurement functions for suitable calibrated microscope images.
The software includes time-lapse imaging functionality for recording changes in specimens over selected observation intervals.
The supplied software configuration lists Z-stack imaging.
This supports acquisition of images at different focus positions when the required focus control and acquisition components are available.
The requested imaging configuration includes image stitching functionality for combining compatible overlapping images.
The software lists AI-assisted image analysis capabilities.
The actual analysis functions depend on the supplied software modules, supported models and validation for the intended research application.
The microscope is specified with an Intel Core i7 computer system for compatible digital imaging workflows.
The supplied workstation configuration includes 8 GB RAM and a 1 TB solid-state drive.
The supplied configuration supports Windows 10 or Windows 11, depending on the installed software and hardware drivers.
The inverted optical arrangement is suitable for observation of compatible cell cultures.
Long-term live-cell imaging may require additional environmental control accessories.
The microscope supports suitable biological research involving fluorescent markers, cell cultures and prepared specimens.
The KCX-166 combines optical observation, digital imaging and software-assisted analysis for advanced laboratory microscopy workflows.
The Inverted Fluorescence Microscope KCX-166 is designed for high-resolution observation of suitable biological specimens using inverted optical microscopy and multiple illumination techniques.
Its inverted design allows specimens to be viewed from beneath the stage, making it particularly useful for examining compatible cell cultures and biological samples in laboratory vessels.
The microscope supports conventional bright-field observation, phase-contrast microscopy, fluorescence imaging and DIC configurations.
Bright-field microscopy provides general transmitted-light observation of suitable specimens.
Phase-contrast microscopy can improve visibility of transparent and unstained specimens when appropriate phase objectives and condenser components are installed.
Fluorescence microscopy allows suitably labelled biological structures to be observed using selected excitation and emission filter arrangements.
DIC microscopy can provide contrast in suitable transparent specimens when the required DIC optical components are installed.
The microscope's digital imaging system allows images to be captured and processed using compatible software.
Time-lapse imaging can support observation of changes in suitable specimens over time.
Z-stack acquisition can support imaging at different focal positions when the required hardware and software functions are available.
The KCX-166 is therefore intended for advanced biological research, fluorescence observation, cell culture studies and scientific image documentation.
The KCX-166 Inverted Fluorescence Microscope is suitable for observing compatible cell cultures and fluorescently labelled cellular structures.
The inverted optical arrangement supports observation of living cells in suitable culture vessels.
Long-duration experiments may require temperature, humidity and gas-control accessories.
The microscope can support observation of fluorescently labelled biological materials used in molecular biology investigations.
Research laboratories can use the microscope for suitable cell-based studies and fluorescence imaging applications related to cancer biology.
The DAPI, FITC, TRITC and CY5 filter configurations support observation of compatible fluorescently labelled specimens.
The microscope can be used for observing suitable microorganisms and prepared biological specimens using compatible microscopy techniques.
The KCX-166 can support observation of suitable stem cell cultures and fluorescently labelled cellular structures.
Pharmaceutical laboratories can use the microscope for appropriate cell-based imaging and fluorescence research applications.
The microscope can support microscopy-based observation in suitable experimental drug discovery workflows.
The KCX-166 is suitable for fluorescence imaging and observation of compatible biological specimens in biotechnology research.
The microscope can support research-oriented examination of suitable prepared tissue specimens, subject to compatibility with the inverted stage and optical arrangement.
Biomedical research institutions can use the microscope for appropriate fluorescence and cell imaging investigations.
The inverted microscope configuration may support suitable reproductive biology research involving compatible specimens and laboratory vessels.
Clinical embryology use requires equipment and procedures validated for that purpose.
Medical universities can use the microscope for advanced biological microscopy education and research.
Research institutes can use the KCX-166 for fluorescence observation, digital imaging and suitable biological experiments.
The microscope may be used for research and method-development activities involving prepared biological specimens.
Clinical diagnostic use requires appropriate validation and regulatory compliance.
The microscope supports observation of compatible fluorescently stained specimens using the installed filter sets.
The digital imaging system supports time-lapse acquisition for suitable experiments.
The microscope can support Z-stack acquisition when the required focusing hardware and software components are installed.
The supplied software configuration supports image measurement and analysis of compatible microscope images.
The Inverted Fluorescence Microscope KCX-166 operates using the principles of inverted optical microscopy, optical magnification, fluorescence excitation and emission, and digital image acquisition.
Unlike upright microscopes, an inverted microscope positions its objective lenses below the specimen stage.
A compatible biological specimen is placed in a suitable culture vessel or laboratory container on the stage.
The microscope objective collects light from the specimen through the bottom of the vessel.
In bright-field mode, transmitted illumination passes through the specimen and is collected by the objective.
In phase-contrast mode, compatible phase optical components convert selected phase differences in transmitted light into visible intensity differences.
In fluorescence mode, excitation light from the fluorescence illumination system is directed toward the specimen through the appropriate filter arrangement.
Fluorescent labels absorb excitation light and emit light at characteristic wavelengths.
The microscope's emission filters help separate the desired fluorescent signal from unwanted excitation illumination.
The resulting image is magnified by the infinity-corrected optical system and observed through the eyepieces or acquired through the digital camera port.
In DIC mode, suitable polarization and interference optics create contrast from optical path differences in compatible specimens.
The digital CMOS camera converts the optical image into electronic image data.
The imaging software can then support live observation, image capture, measurement and compatible advanced imaging functions.
Specimen Preparation: A compatible biological specimen is prepared using suitable fluorescent labels.
Specimen Placement: The specimen is positioned in a compatible vessel on the mechanical stage.
Excitation Illumination: The fluorescence system produces light for the selected fluorescence channel.
Optical Filtering: The excitation filter selects suitable wavelengths.
Fluorescence Emission: Fluorescent molecules emit light after excitation.
Image Formation: The objective and infinity-corrected optical system form a magnified image.
Digital Acquisition: The scientific CMOS camera captures the optical image.
Image Processing: The imaging software supports compatible documentation and analysis functions.
| Objective | Eyepiece | Nominal Optical Magnification |
|---|---|---|
| 10X Long Working Distance | WF10X | 100X |
| 20X Long Working Distance | WF10X | 200X |
| 40X Long Working Distance | WF10X | 400X |
| 60X Long Working Distance | WF10X | 600X |
The listed magnifications are calculated from the supplied objective and eyepiece values. Actual optical performance depends on objective specifications, numerical aperture and the final microscope configuration.
| Filter Set | Intended Fluorescence Channel |
|---|---|
| DAPI | DAPI-Compatible Fluorescence |
| FITC | FITC-Compatible Fluorescence |
| TRITC | TRITC-Compatible Fluorescence |
| CY5 | CY5-Compatible Fluorescence |
The exact excitation and emission wavelengths must be confirmed from the installed filter-set specifications.
| Parameter | Specification |
|---|---|
| Camera Type | Scientific CMOS |
| Specified Camera Resolution | 25 MP |
| Specified Image Resolution | 4800 × 3600 Pixels |
| Maximum Frame Rate | Up to 60 fps |
| USB Interface | USB 3.2 Gen 2 |
| Display Interface | HDMI |
| Wireless Connectivity | Wi-Fi |
| Imaging Software | Scientific Imaging and Measurement |
| Time-Lapse Imaging | Supported |
| Z-Stack Imaging | Configuration-Dependent |
| Image Stitching | Software-Dependent |
| AI-Assisted Analysis | Software-Dependent |
| Computer | Intel Core i7 |
| RAM | 8 GB |
| Storage | 1 TB SSD |
| Operating System | Windows 10 / Windows 11 |
The KCX-166 Inverted Fluorescence Microscope is designed for observation of suitable biological specimens, cell cultures and fluorescently labelled samples.
It can be used in biotechnology laboratories, biomedical research centers, universities, pharmaceutical research facilities and scientific institutions.
For routine observation, the specimen should be placed in a compatible laboratory vessel on the mechanical stage.
The appropriate objective lens should be selected according to the specimen and observation requirements.
The white LED illumination system can be used for compatible transmitted-light observation.
For fluorescence imaging, the selected fluorescent label must be compatible with the installed excitation and emission filter arrangement.
Phase-contrast observation requires suitable phase objectives and condenser components.
DIC observation requires the appropriate DIC optical accessories.
The integrated camera system can be used for image acquisition and documentation through compatible software.
For extended live-cell imaging, suitable environmental control equipment may be required to maintain specimen conditions.
The microscope should be operated according to its final supplied technical configuration and laboratory safety instructions.
