INVERTED PHASE CONTRAST KCX-171

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• 1. Advanced Inverted Phase Contrast Microscope
• The KCX-171 Inverted Phase Contrast Microscope is designed for direct observation of compatible cell cultures and biological specimens.
• Its inverted configuration positions the objective lenses below the specimen stage.
• 2. Tissue Culture Microscope Configuration
• The KCX-171 is intended for tissue culture and cell culture observation in suitable laboratory vessels.
• This configuration reduces the need to transfer specimens onto conventional microscope slides for routine observation.
• 3. Infinity Plan Optical System
• The microscope incorporates an infinity plan optical system designed to support clear magnified images and its specified optical accessories.
• 4. Bright-Field Observation
• The KCX-171 supports conventional bright-field observation using transmitted illumination.
• This mode is useful for examining suitable prepared biological specimens.
• 5. Phase-Contrast Observation
• The microscope includes a phase-contrast system designed to enhance the visibility of transparent and low-contrast specimens.
• Phase contrast is particularly useful for examining suitable living and unstained cells.
• 6. Long Working Distance Objectives
• The microscope incorporates long working distance objectives intended for observation through compatible culture vessel bottoms.
• 7. Semi-Apochromatic Optical Configuration
• The specified LWD Semi-APO FL objectives are designed to provide improved optical correction compared with simpler objective configurations.
• 8. 10X, 20X and 40X Objectives
• The supplied infinity plan objective configuration includes 10X, 20X and 40X magnifications.
• 9. Dedicated 20X Phase Contrast Objective
• The KCX-171 includes an Infinity Phase Contrast LWD Semi-APO FL 20X objective for compatible phase-contrast observation.
• 10. 100X–400X Magnification
• The microscope provides nominal magnifications of 100X, 200X and 400X using the supplied WF10X eyepieces and listed objectives.
• 11. Seidentopf Trinocular Head
• The microscope features a Seidentopf trinocular viewing head for binocular observation and compatible camera attachment.
• 12. Ergonomic 45° Inclined Head
• The KCX-171 incorporates a 45° inclined observation head, providing a practical viewing angle for laboratory work.
• 13. 360° Rotating Eyetube Assembly
• The viewing assembly is specified with 360° rotation, supporting adjustment of the observation orientation.
• 14. Adjustable Interpupillary Distance
• The microscope supports an interpupillary adjustment range of 48–76 mm.
• 15. Dual Diopter Adjustment
• Both eyepiece tubes include diopter adjustment to accommodate differences between the user's eyes.
• 16. Wide-Field WF10X / 22 mm Eyepieces
• The microscope includes two WF10X / 22 mm wide-field eyepieces.
• 17. Five-Position Revolving Nosepiece
• The KCX-171 incorporates a five-position revolving nosepiece for selecting the installed objective lenses.
• 18. Selectable Trinocular Light Path
• The microscope supports E100 and E20 optical light-path configurations.
• These settings allow light distribution between the eyepiece and camera paths according to the selected position.
• 19. Large 210 × 241 mm Working Stage
• The microscope features a large stage designed to accommodate suitable culture vessels and specimen holders.
• 20. Attachable Stage Components
• The working stage supports compatible attachable components for different observation requirements.
• 21. 54 mm Culture Dish Holder
• The supplied reference includes a 54 mm culture dish holder.
• 22. Ø110 mm Round Slide Accommodation
• The microscope includes provision for a 110 mm diameter round slide or compatible stage insert, as specified in the reference.
• 23. LWD / ELWD Condenser
• The KCX-171 incorporates a long working distance or extra-long working distance condenser arrangement.
• 24. N.A. 0.3 Condenser
• The condenser has a specified numerical aperture of 0.3.
• 25. Extended Condenser Working Distance
• The supplied reference specifies a condenser working distance of 72 mm with the condenser and 195 mm without it.
• 26. Phase Contrast Annular Spots
• The phase-contrast configuration lists annular spot arrangements for 10X and 20X/40X observation.
• The appropriate annulus must be matched to the installed phase objective.
• 27. Phase Contrast Slide Slot
• The condenser arrangement includes a dedicated slot for compatible phase-contrast slides.
• 28. Köhler Illumination
• The microscope features Köhler illumination for controlled transmitted-light observation.
• 29. 6V / 30W Halogen Lamp
• The KCX-171 incorporates a 6V / 30W halogen transmitted-light illumination source.
• 30. Blue and Green Filters
• The supplied configuration includes blue and green filters with Ø34 mm diameter.
• 31. Coaxial Coarse and Fine Focusing
• The microscope features coaxial focusing controls for convenient initial and precise focus adjustment.
• 32. 0.002 mm Fine Focusing Scale
• The KCX-171 includes a specified fine focusing scale of 0.002 mm, equivalent to 2 micrometers.
• 33. Adjustable Focusing Tension
• The focusing mechanism includes adjustable tension for controlled operation.
• 34. Digital Camera Compatibility
• The trinocular observation head supports integration with a compatible digital microscope camera.
• 35. Wide-Range Power Supply
• The microscope supports AC 110–240V electrical input.
• 36. Optional Fluorescence Configuration
• The supplied reference identifies fluorescence as a supported configuration.
• Fluorescence observation requires compatible illumination and optical filter components.
• 37. Optional Reflected-Light Configuration
• The microscope reference also lists a reflected-light configuration.
• The required components depend on the selected system.
• 38. Live-Cell Observation
• The inverted phase-contrast configuration supports observation of compatible living cell cultures.
• 39. Biotechnology Research Applications
• The KCX-171 is suitable for biological microscopy workflows in biotechnology research laboratories.
• 40. Laboratory Research and Education
• The microscope supports tissue culture education, cell observation and advanced life science laboratory training.

The Inverted Phase Contrast Microscope KCX-171 is designed primarily for tissue culture, cell culture and live-cell observation.

Its inverted optical configuration positions the objective lenses below the specimen stage.

This allows researchers to observe suitable biological specimens through the transparent bottom of compatible culture dishes, flasks and other laboratory vessels.

The microscope supports both bright-field and phase-contrast observation.

Bright-field microscopy is useful for general observation of suitably prepared specimens.

Phase-contrast microscopy improves the visibility of transparent and low-contrast specimens by converting optical phase differences into visible intensity variations.

This makes phase contrast useful for examining suitable living cells without routine staining.

The microscope's long working distance objectives provide clearance for compatible culture vessels.

The mechanical stage and attachable holders support specimen positioning.

The trinocular observation head allows a compatible camera to be attached for image capture and documentation.

The KCX-171 is therefore intended for cell culture monitoring, tissue culture research, cell morphology examination, biotechnology studies and advanced biological microscopy education.

Applications

1. Tissue Culture Laboratories

The KCX-171 Inverted Phase Contrast Microscope is suitable for routine observation of compatible tissue culture specimens.

2. Cell Culture Laboratories

The microscope supports observation of living cells in suitable culture dishes and flasks.

3. Cell Biology Research

Researchers can use the microscope to examine cellular morphology and other visible characteristics.

4. Live-Cell Observation

The inverted phase-contrast configuration is useful for observing suitable living cells without routine staining.

5. Biotechnology Research

The microscope supports microscopy-based observation in biotechnology laboratories.

6. Molecular Biology Research

The KCX-171 can support examination of suitable biological specimens used in molecular biology studies.

7. Microbiology Laboratories

The microscope may be used for observation of compatible microorganisms and cell cultures.

8. Pharmaceutical Research

Pharmaceutical laboratories can use the microscope for suitable cell-based research and observation workflows.

9. Drug Discovery Research

The microscope can support observation of cell cultures used in appropriate drug discovery studies.

10. Cancer Research

Research laboratories can use the KCX-171 for suitable cell culture observation associated with cancer biology investigations.

11. Stem Cell Research

The microscope supports observation of compatible stem cell cultures and cellular morphology.

12. IVF and Reproductive Biology Research

The inverted microscope configuration may support suitable reproductive biology research involving compatible specimens.

Clinical embryology use requires appropriate validation and specialized operating procedures.

13. Immunology Research

The microscope can support observation of suitable cell cultures used in immunology research.

14. Virology Research

The KCX-171 can support observation of compatible cell cultures used in virology research workflows.

15. Biomedical Research

Biomedical laboratories can use the microscope for appropriate cell-based observation and documentation.

16. Life Science Laboratories

The microscope is suitable for biological microscopy in life science research and teaching laboratories.

17. Medical Research Institutions

Medical research institutions can use the KCX-171 for research-oriented observation of suitable cell cultures.

18. Universities and Colleges

Universities can use the microscope for tissue culture education, cell biology practicals and laboratory research.

19. Research Institutes

Scientific institutions can use the microscope for advanced cell observation and phase-contrast microscopy.

20. Cell Culture Monitoring

The microscope can support visual monitoring of cell morphology, density and visible culture characteristics.

Typical Observation and Research Areas

Working Principle

The Inverted Phase Contrast Microscope KCX-171 operates using the principles of inverted optical microscopy, transmitted-light illumination and phase-contrast image formation.

Unlike conventional upright microscopes, an inverted microscope positions its objective lenses beneath the specimen stage.

A compatible culture vessel containing the specimen is placed on the microscope stage.

Light from the 6V / 30W halogen illumination system passes through the condenser and specimen.

The objective lens beneath the specimen collects the transmitted light.

In bright-field observation, differences in specimen absorption and scattering produce visible image contrast.

In phase-contrast observation, the condenser annulus and compatible phase objective work together to convert optical phase differences into visible intensity differences.

This improves the visibility of many transparent and low-contrast specimens.

The microscope's infinity plan optical system forms a magnified image for observation through the eyepieces.

The trinocular head can also direct light toward a compatible camera for digital imaging.

The coaxial coarse and fine focusing controls allow the user to adjust image sharpness.

Phase Contrast Observation Process

1. Specimen Preparation: A suitable biological specimen is prepared in a compatible culture vessel.

2. Specimen Placement: The vessel is positioned on the working stage using an appropriate holder.

3. Illumination: The transmitted halogen light source provides illumination through the condenser.

4. Objective Selection: The required long working distance objective is selected.

5. Phase Annulus Selection: The matching phase-contrast annulus is selected for phase observation.

6. Focusing: Coarse and fine focusing controls are adjusted to obtain a clear image.

7. Image Observation: The specimen is examined through the eyepieces.

8. Digital Documentation: A compatible camera may be used for image capture when installed.

Magnification System

The microscope's standard nominal optical magnification range is 100X–400X.

Phase Contrast Optical System

Stage and Culture Vessel Compatibility

The compatibility of individual culture vessels depends on their dimensions, bottom thickness, optical properties and the installed holder.

Digital Imaging Compatibility

Microscope Compatibility and Usage

The KCX-171 Inverted Phase Contrast Microscope is designed for observation of suitable cell cultures, tissue culture samples and other biological specimens.

It can be used in cell culture laboratories, biotechnology research centers, universities, pharmaceutical research facilities and scientific institutions.

For routine observation, the specimen should be placed in a compatible culture vessel on the working stage.

The appropriate long working distance objective should be selected according to the specimen and vessel configuration.

Bright-field observation can be used for general specimen examination.

For phase-contrast observation, the appropriate phase objective and matching condenser annulus must be selected and aligned.

The microscope's focusing controls allow adjustment of image sharpness.

The trinocular port can be used for compatible digital camera integration.

For extended live-cell observation, additional environmental control accessories may be necessary to maintain suitable specimen conditions.

The microscope should be operated according to its final supplied technical configuration and laboratory safety instructions.