The Inverted Tissue Culture Microscope KCX-173 is designed for observing suitable living cells, tissue cultures, microorganisms and transparent biological specimens.
Unlike conventional upright microscopes, its inverted configuration positions the objective lenses beneath the specimen stage.
This arrangement allows specimens to be examined through the transparent bottom of compatible culture dishes, flasks and other laboratory vessels.
The microscope supports bright-field and phase-contrast observation.
Bright-field microscopy is useful for general observation of suitably prepared biological specimens.
Phase-contrast microscopy enhances the visibility of transparent and low-contrast specimens by converting optical phase differences into visible intensity variations.
The microscope's infinity plan achromatic objectives provide different magnification options for suitable biological observation.
The dedicated PH10X, PH20X and PH40X objectives support phase-contrast imaging when used with correctly aligned matching phase annuli.
The large working stage supports positioning of compatible laboratory culture vessels.
The super long working distance condenser provides clearance for suitable culture arrangements.
The microscope's coaxial focusing mechanism allows controlled adjustment of image sharpness.
The KCX-173 is therefore intended for tissue culture observation, cell biology research, biotechnology studies and biological microscopy education.
Applications
1. Tissue Culture Laboratories
The KCX-173 Inverted Tissue Culture Microscope is suitable for observation of compatible tissue culture specimens.
2. Living Cell Observation
The microscope supports observation of suitable living cells in compatible culture vessels.
3. Cell Biology Research
Researchers can use the microscope to examine cell morphology and visible cellular characteristics.
4. Microbiology Studies
The KCX-173 supports observation of suitable microorganisms and biological specimens.
5. Biotechnology Research
The microscope is suitable for cell-based observation and microscopy applications in biotechnology laboratories.
6. Pharmaceutical Research
Pharmaceutical laboratories can use the microscope for appropriate biological research workflows.
7. Cell Culture Studies
The KCX-173 supports routine visual examination of compatible cell cultures.
8. Molecular Biology Research
The microscope can assist in observation of suitable specimens used in molecular biology studies.
9. Cancer Research
Research laboratories can use the microscope for suitable cell culture observation associated with cancer biology studies.
10. Stem Cell Research
The microscope supports observation of compatible stem cell cultures.
11. Drug Discovery Research
The KCX-173 can support microscopy-based observation of cell cultures in appropriate drug discovery studies.
12. Biomedical Research
Biomedical laboratories can use the microscope for suitable cell observation and documentation.
13. Life Science Laboratories
The microscope is suitable for biological microscopy in life science research environments.
14. Medical Research Institutions
Medical research institutions can use the KCX-173 for research-oriented observation of compatible biological specimens.
15. Universities and Colleges
The microscope supports cell biology practicals, tissue culture training and phase-contrast microscopy education.
16. Scientific Research Institutes
Research institutes can use the microscope for advanced biological observation.
17. Cell Morphology Examination
The microscope supports examination of visible cellular shape and structural characteristics.
18. Cell Growth Monitoring
The KCX-173 can support visual observation of changes in suitable cell cultures.
19. Cell Attachment Observation
The microscope can assist in observing attachment characteristics of compatible adherent cells.
20. Cell Culture Monitoring
The microscope supports qualitative assessment of visible culture characteristics.
Advanced Phase Contrast Observation
The KCX-173 Inverted Tissue Culture Microscope incorporates a dedicated phase-contrast system for observation of transparent and low-contrast specimens.
Phase-contrast microscopy is particularly useful for examining living cells that may be difficult to distinguish under conventional bright-field conditions.
The microscope includes PH10X, PH20X and PH40X infinity plan phase objectives.
A center-adjustable phase-contrast sliding plate supports matching annular illumination configurations.
The supplied Ø30 mm centering telescope assists with alignment of the phase-contrast system.
Proper phase-contrast observation requires the selected objective to be used with the corresponding phase annulus.
This arrangement supports observation of compatible living cells, tissue cultures and biological specimens.
Inverted Optical Design
The KCX-173 uses an inverted microscope configuration with the objective lenses positioned beneath the specimen stage.
This design is useful for tissue culture applications because specimens can be observed directly through compatible transparent culture vessel bottoms.
The inverted configuration provides access to the specimen area from above.
Its long working distance optical components are intended for observation through suitable culture vessels.
The supplied reference specifies up to 150 mm culture vessel clearance when the condenser is removed.
Actual vessel compatibility depends on dimensions, bottom thickness and the installed optical arrangement.
Optical Performance
The KCX-173 features an infinity plan achromatic optical system.
The supplied objective configuration includes 4X, 10X, 20X and 40X bright-field objectives, together with PH10X, PH20X and PH40X phase-contrast objectives.
The EW10X / 22 mm extra-wide-field eyepieces provide a wide viewing field.
The microscope supports nominal optical magnification from 40X to 400X.
The actual image quality and resolution depend on the installed objective, numerical aperture, specimen preparation and illumination configuration.
Working Principle
The Inverted Tissue Culture Microscope KCX-173 operates using the principles of inverted optical microscopy, transmitted-light illumination and phase-contrast image formation.
A compatible biological specimen is placed in a suitable culture vessel on the microscope stage.
The 6V / 30W halogen illumination system provides light that passes through the condenser and specimen.
The objective lens beneath the specimen collects transmitted light.
In bright-field observation, differences in light absorption and scattering create visible image contrast.
In phase-contrast observation, the condenser annulus and compatible phase objective work together to convert phase differences into visible intensity variations.
This improves the visibility of suitable transparent and low-contrast biological specimens.
The infinity-corrected optical system forms a magnified image for observation through the eyepieces.
The coaxial coarse and fine focusing controls allow the user to adjust image sharpness.
Phase Contrast Observation Process
Specimen Placement: A compatible culture vessel containing the specimen is positioned on the microscope stage.
Illumination: The halogen light source provides transmitted illumination.
Objective Selection: The required bright-field or phase-contrast objective is selected.
Phase Alignment: The matching phase annulus is selected and aligned using the appropriate adjustment controls.
Focusing: Coarse and fine focusing controls are adjusted.
Image Observation: The specimen is examined through the eyepieces.
Documentation: Observations may be recorded using a compatible imaging arrangement where available.
Magnification System
| Objective | Eyepiece | Total Optical Magnification |
|---|
| Infinity Plan 4X / 0.10 | EW10X | 40X |
| Infinity Plan 10X / 0.25 | EW10X | 100X |
| Infinity Plan 20X / 0.40 | EW10X | 200X |
| Infinity Plan 40X / 0.60 | EW10X | 400X |
| Infinity Plan PH10X / 0.25 | EW10X | 100X |
| Infinity Plan PH20X / 0.40 | EW10X | 200X |
| Infinity Plan PH40X / 0.60 | EW10X | 400X |
The standard nominal magnification range is 40X–400X.
Phase Contrast Optical System
| Parameter | Specification |
|---|
| Observation Mode | Phase Contrast |
| Optical System | Infinity Plan |
| Phase Objectives | PH10X, PH20X and PH40X |
| Phase Attachment | Center-Adjustable Sliding Plate |
| Phase Annulus Configurations | 10X and 20X–40X |
| Centering Telescope | Ø30 mm |
| Condenser | Super Long Working Distance |
| Condenser N.A. | 0.3 |
| Condenser Working Distance | 72 mm |
| Illumination | 6V / 30W Halogen |
| Main Purpose | Observation of Transparent and Low-Contrast Biological Specimens |
Stage and Culture Vessel Compatibility
| Parameter | Specification |
|---|
| Stage Type | Large Plane Stage |
| Stage Dimensions | 160 × 250 mm |
| Condenser Working Distance | 72 mm |
| Clearance Without Condenser | Up to 150 mm |
| Compatible Specimens | Suitable Culture Dishes, Flasks and Laboratory Vessels |
| Observation Arrangement | Inverted |
| Specimen Access | From Above the Stage |
Compatibility with individual culture vessels depends on the vessel dimensions, bottom thickness and optical properties.
Digital Imaging Compatibility
| Parameter | Specification |
|---|
| Viewing Head Options | Binocular / Trinocular |
| Camera Attachment | Supported with Trinocular Configuration |
| Camera Resolution | Not Specified |
| Image Capture | Depends on Installed Camera |
| Video Recording | Depends on Installed Camera |
| Imaging Software | Not Specified |
| Computer Connectivity | Depends on Selected Camera System |
Microscope Compatibility and Usage
The KCX-173 Inverted Tissue Culture Microscope is designed for observation of suitable tissue cultures, cell cultures and biological specimens.
It can be used in biotechnology laboratories, microbiology facilities, pharmaceutical research centers, universities and scientific institutions.
For routine observation, the specimen should be placed in a compatible culture vessel on the microscope stage.
The appropriate bright-field or phase-contrast objective should be selected according to the specimen.
For phase-contrast observation, the corresponding phase annulus should be aligned correctly.
The microscope's focusing controls allow adjustment of image sharpness.
The halogen illumination system provides transmitted light for observation.
The optical filters can be used according to the selected illumination requirements.
For extended live-cell observation, additional environmental control equipment may be necessary.
The microscope should be operated according to its final supplied technical configuration and applicable laboratory safety procedures.