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INVERTED TISSUE CULTURE MICROSCOPE KCX-173
Microscope / Inverted Microscopes

INVERTED TISSUE CULTURE MICROSCOPE KCX-173

The Inverted Tissue Culture Microscope KCX-173 by KC SCIENTIFIC WORKS features infinity plan achromatic optics, phase-contrast objectives, extra-wide-field eyepieces, a large working stage and halogen illumination. Designed for cell culture, tissue culture and biotechnology research, it supports 40X–400X magnification and observation of compatible living biological specimens in laboratory vessels.

SKU: KCX-173

Category: Microscope, Inverted Microscopes

Description

Contact our team for a detailed product description.

Technical Specifications

ParameterSpecification
Product NameInverted Tissue Culture Microscope with Phase Contrast Attachment
Model NumberKCX-173
BrandKC SCIENTIFIC WORKS
ManufacturerKC SCIENTIFIC WORKS
Microscope TypeInverted Tissue Culture / Phase Contrast Microscope
Optical SystemInfinity Plan Achromatic
Observation ModesBright Field and Phase Contrast
Viewing HeadCompensation-Free Binocular / Trinocular
Head Inclination30°
Head Rotation360° Rotatable
Interpupillary Distance48–75 mm
EyepiecesHigh-Point Extra Wide Field EW10X / 22 mm
NosepieceInverse Quintuple Revolving Nosepiece
Bright-Field ObjectivesInfinity Plan Achromatic 4X, 10X, 20X and 40X
Phase Contrast ObjectivesInfinity Plan PH10X, PH20X and PH40X
4X Objective4X / N.A. 0.10, WD 18 mm
10X Objective10X / N.A. 0.25, WD 10 mm
20X Objective20X / N.A. 0.40, WD 5.1 mm
40X Objective40X / N.A. 0.60, WD 2.6 mm, Cover Slip 1.2 mm
PH10X ObjectivePH10X / N.A. 0.25, WD 10 mm
PH20X ObjectivePH20X / N.A. 0.40, WD 5.1 mm
PH40X ObjectivePH40X / N.A. 0.60, WD 0.35 mm, Cover Slip 1.2 mm
Nominal Magnification Range40X–400X
Condenser TypeSuper Long Working Distance Condenser
Condenser Numerical ApertureN.A. 0.3
Condenser Working Distance72 mm
Culture Vessel ClearanceUp to 150 mm with Condenser Removed
Centering TelescopeØ30 mm
Stage TypeLarge Plane Stage
Stage Dimensions160 × 250 mm
Phase Contrast AttachmentCenter-Adjustable Sliding Plate
Phase Contrast Sliding Plate10X and 20X–40X Configurations
Focusing SystemCoaxial Coarse and Fine Focusing
Focusing MechanismRack and Pinion
Fine Focusing Scale0.002 mm
Illumination TypeTransmitted Light
Light SourceHalogen Lamp
Lamp Rating6V / 30W
Optical FiltersBlue, Green, Yellow and Frosted Glass
Camera CompatibilityTrinocular Configuration Supports Compatible Camera Attachment
Main ApplicationsTissue Culture, Cell Biology, Microbiology, Biotechnology and Research
Product CategoryInverted Tissue Culture and Phase Contrast Microscopes

Key Features

  • 1. Advanced Inverted Tissue Culture Microscope
  • The KCX-173 Inverted Tissue Culture Microscope is designed for observing suitable living cells, tissue cultures and biological specimens directly in compatible laboratory vessels.
  • Its inverted optical configuration places the objective lenses beneath the specimen stage.
  • 2. Infinity Plan Achromatic Optical System
  • The microscope incorporates an infinity plan achromatic optical system designed to provide magnified observation of suitable biological specimens.
  • The optical configuration supports compatible bright-field and phase-contrast objectives.
  • 3. Bright-Field Observation
  • The KCX-173 supports bright-field microscopy for general examination of suitable biological specimens.
  • 4. Advanced Phase Contrast Observation
  • The microscope incorporates dedicated phase-contrast objectives and a compatible phase-contrast attachment.
  • This observation method improves the visibility of transparent and low-contrast specimens.
  • 5. Suitable for Living Cell Observation
  • The inverted phase-contrast configuration supports observation of compatible living cells without requiring routine staining for basic morphological examination.
  • 6. Compensation-Free Viewing Head
  • The microscope offers a compensation-free binocular or trinocular viewing head, depending on the selected configuration.
  • 7. Ergonomic 30° Inclined Head
  • The 30° inclined observation head provides a convenient viewing angle for laboratory microscopy.
  • 8. 360° Rotatable Viewing Head
  • The observation head supports 360° rotation, allowing adjustment of the viewing orientation.
  • 9. Adjustable Interpupillary Distance
  • The microscope provides an interpupillary adjustment range of 48–75 mm.
  • 10. Extra-Wide-Field Eyepieces
  • The KCX-173 features EW10X / 22 mm high-point eyepieces for wide-field optical observation.
  • 11. Inverse Quintuple Revolving Nosepiece
  • The microscope incorporates a five-position inverted nosepiece for selecting compatible objective lenses.
  • 12. Infinity Plan Achromatic 4X Objective
  • The supplied reference includes a 4X / N.A. 0.10 objective with an 18 mm working distance.
  • 13. Infinity Plan Achromatic 10X Objective
  • The microscope includes a 10X / N.A. 0.25 objective with a 10 mm working distance.
  • 14. Infinity Plan Achromatic 20X Objective
  • The KCX-173 includes a 20X / N.A. 0.40 objective with a 5.1 mm working distance.
  • 15. Infinity Plan Achromatic 40X Objective
  • The microscope includes a 40X / N.A. 0.60 objective with a 2.6 mm working distance and a specified 1.2 mm cover-slip parameter.
  • 16. Dedicated PH10X Objective
  • The phase-contrast configuration includes a PH10X / N.A. 0.25 objective with a 10 mm working distance.
  • 17. Dedicated PH20X Objective
  • The microscope includes a PH20X / N.A. 0.40 objective with a 5.1 mm working distance.
  • 18. Dedicated PH40X Objective
  • The KCX-173 includes a PH40X / N.A. 0.60 objective with a 0.35 mm working distance and a specified 1.2 mm cover-slip parameter.
  • 19. 40X–400X Magnification Range
  • The microscope provides nominal optical magnifications of 40X, 100X, 200X and 400X using the specified 10X eyepieces and corresponding objectives.
  • 20. Super Long Working Distance Condenser
  • The microscope features a super long working distance condenser designed for inverted microscopy applications.
  • 21. Condenser Numerical Aperture of 0.3
  • The condenser has a specified numerical aperture of 0.3.
  • 22. 72 mm Condenser Working Distance
  • The KCX-173 provides a specified condenser working distance of 72 mm.
  • 23. Extended Culture Vessel Clearance
  • The supplied reference specifies clearance for compatible culture vessels up to 150 mm high when the condenser is removed.
  • 24. Large 160 × 250 mm Stage
  • The microscope features a 160 × 250 mm plane stage for positioning compatible culture vessels and specimens.
  • 25. Phase Contrast Sliding Plate
  • The KCX-173 incorporates a phase-contrast sliding plate with configurations for 10X and 20X–40X objectives.
  • 26. Center-Adjustable Phase Contrast System
  • The phase-contrast attachment includes centering adjustment to support proper alignment of compatible phase optical components.
  • 27. Ø30 mm Centering Telescope
  • The microscope includes a specified 30 mm diameter centering telescope for phase-contrast alignment.
  • 28. Coaxial Coarse and Fine Focusing
  • The microscope features coaxial coarse and fine focusing controls for convenient image adjustment.
  • 29. Rack-and-Pinion Focusing Mechanism
  • The KCX-173 incorporates a rack-and-pinion focusing mechanism.
  • 30. Fine Focusing Scale of 0.002 mm
  • The microscope provides a fine focusing scale of 0.002 mm, equivalent to 2 micrometers.
  • 31. 6V / 30W Halogen Illumination
  • The KCX-173 features a 6V / 30W halogen light source for transmitted-light microscopy.
  • 32. Blue Optical Filter
  • The supplied configuration includes a blue glass filter for compatible illumination adjustment.
  • 33. Green Optical Filter
  • The microscope includes a green glass filter for suitable observation requirements.
  • 34. Yellow Optical Filter
  • The KCX-173 includes a yellow glass filter.
  • 35. Frosted Glass Filter
  • The microscope includes a frosted glass filter for suitable illumination applications.
  • 36. Digital Imaging Compatibility
  • The trinocular configuration supports connection to a compatible microscope camera.
  • 37. Cell Culture Observation
  • The microscope is designed for observation of compatible cell cultures in suitable laboratory vessels.
  • 38. Tissue Culture Research
  • The KCX-173 supports microscopic observation of suitable tissue culture specimens.
  • 39. Biotechnology Applications
  • The microscope is suitable for appropriate microscopy workflows in biotechnology research laboratories.
  • 40. Laboratory Research and Education
  • The KCX-173 supports biological microscopy education, phase-contrast training and laboratory research applications.

Working Purpose

The Inverted Tissue Culture Microscope KCX-173 is designed for observing suitable living cells, tissue cultures, microorganisms and transparent biological specimens.

Unlike conventional upright microscopes, its inverted configuration positions the objective lenses beneath the specimen stage.

This arrangement allows specimens to be examined through the transparent bottom of compatible culture dishes, flasks and other laboratory vessels.

The microscope supports bright-field and phase-contrast observation.

Bright-field microscopy is useful for general observation of suitably prepared biological specimens.

Phase-contrast microscopy enhances the visibility of transparent and low-contrast specimens by converting optical phase differences into visible intensity variations.

The microscope's infinity plan achromatic objectives provide different magnification options for suitable biological observation.

The dedicated PH10X, PH20X and PH40X objectives support phase-contrast imaging when used with correctly aligned matching phase annuli.

The large working stage supports positioning of compatible laboratory culture vessels.

The super long working distance condenser provides clearance for suitable culture arrangements.

The microscope's coaxial focusing mechanism allows controlled adjustment of image sharpness.

The KCX-173 is therefore intended for tissue culture observation, cell biology research, biotechnology studies and biological microscopy education.

Applications

1. Tissue Culture Laboratories

The KCX-173 Inverted Tissue Culture Microscope is suitable for observation of compatible tissue culture specimens.

2. Living Cell Observation

The microscope supports observation of suitable living cells in compatible culture vessels.

3. Cell Biology Research

Researchers can use the microscope to examine cell morphology and visible cellular characteristics.

4. Microbiology Studies

The KCX-173 supports observation of suitable microorganisms and biological specimens.

5. Biotechnology Research

The microscope is suitable for cell-based observation and microscopy applications in biotechnology laboratories.

6. Pharmaceutical Research

Pharmaceutical laboratories can use the microscope for appropriate biological research workflows.

7. Cell Culture Studies

The KCX-173 supports routine visual examination of compatible cell cultures.

8. Molecular Biology Research

The microscope can assist in observation of suitable specimens used in molecular biology studies.

9. Cancer Research

Research laboratories can use the microscope for suitable cell culture observation associated with cancer biology studies.

10. Stem Cell Research

The microscope supports observation of compatible stem cell cultures.

11. Drug Discovery Research

The KCX-173 can support microscopy-based observation of cell cultures in appropriate drug discovery studies.

12. Biomedical Research

Biomedical laboratories can use the microscope for suitable cell observation and documentation.

13. Life Science Laboratories

The microscope is suitable for biological microscopy in life science research environments.

14. Medical Research Institutions

Medical research institutions can use the KCX-173 for research-oriented observation of compatible biological specimens.

15. Universities and Colleges

The microscope supports cell biology practicals, tissue culture training and phase-contrast microscopy education.

16. Scientific Research Institutes

Research institutes can use the microscope for advanced biological observation.

17. Cell Morphology Examination

The microscope supports examination of visible cellular shape and structural characteristics.

18. Cell Growth Monitoring

The KCX-173 can support visual observation of changes in suitable cell cultures.

19. Cell Attachment Observation

The microscope can assist in observing attachment characteristics of compatible adherent cells.

20. Cell Culture Monitoring

The microscope supports qualitative assessment of visible culture characteristics.

Advanced Phase Contrast Observation

The KCX-173 Inverted Tissue Culture Microscope incorporates a dedicated phase-contrast system for observation of transparent and low-contrast specimens.

Phase-contrast microscopy is particularly useful for examining living cells that may be difficult to distinguish under conventional bright-field conditions.

The microscope includes PH10X, PH20X and PH40X infinity plan phase objectives.

A center-adjustable phase-contrast sliding plate supports matching annular illumination configurations.

The supplied Ø30 mm centering telescope assists with alignment of the phase-contrast system.

Proper phase-contrast observation requires the selected objective to be used with the corresponding phase annulus.

This arrangement supports observation of compatible living cells, tissue cultures and biological specimens.

Inverted Optical Design

The KCX-173 uses an inverted microscope configuration with the objective lenses positioned beneath the specimen stage.

This design is useful for tissue culture applications because specimens can be observed directly through compatible transparent culture vessel bottoms.

The inverted configuration provides access to the specimen area from above.

Its long working distance optical components are intended for observation through suitable culture vessels.

The supplied reference specifies up to 150 mm culture vessel clearance when the condenser is removed.

Actual vessel compatibility depends on dimensions, bottom thickness and the installed optical arrangement.

Optical Performance

The KCX-173 features an infinity plan achromatic optical system.

The supplied objective configuration includes 4X, 10X, 20X and 40X bright-field objectives, together with PH10X, PH20X and PH40X phase-contrast objectives.

The EW10X / 22 mm extra-wide-field eyepieces provide a wide viewing field.

The microscope supports nominal optical magnification from 40X to 400X.

The actual image quality and resolution depend on the installed objective, numerical aperture, specimen preparation and illumination configuration.

Working Principle

The Inverted Tissue Culture Microscope KCX-173 operates using the principles of inverted optical microscopy, transmitted-light illumination and phase-contrast image formation.

A compatible biological specimen is placed in a suitable culture vessel on the microscope stage.

The 6V / 30W halogen illumination system provides light that passes through the condenser and specimen.

The objective lens beneath the specimen collects transmitted light.

In bright-field observation, differences in light absorption and scattering create visible image contrast.

In phase-contrast observation, the condenser annulus and compatible phase objective work together to convert phase differences into visible intensity variations.

This improves the visibility of suitable transparent and low-contrast biological specimens.

The infinity-corrected optical system forms a magnified image for observation through the eyepieces.

The coaxial coarse and fine focusing controls allow the user to adjust image sharpness.

Phase Contrast Observation Process

  1. Specimen Placement: A compatible culture vessel containing the specimen is positioned on the microscope stage.

  2. Illumination: The halogen light source provides transmitted illumination.

  3. Objective Selection: The required bright-field or phase-contrast objective is selected.

  4. Phase Alignment: The matching phase annulus is selected and aligned using the appropriate adjustment controls.

  5. Focusing: Coarse and fine focusing controls are adjusted.

  6. Image Observation: The specimen is examined through the eyepieces.

  7. Documentation: Observations may be recorded using a compatible imaging arrangement where available.

Magnification System

ObjectiveEyepieceTotal Optical Magnification
Infinity Plan 4X / 0.10EW10X40X
Infinity Plan 10X / 0.25EW10X100X
Infinity Plan 20X / 0.40EW10X200X
Infinity Plan 40X / 0.60EW10X400X
Infinity Plan PH10X / 0.25EW10X100X
Infinity Plan PH20X / 0.40EW10X200X
Infinity Plan PH40X / 0.60EW10X400X

The standard nominal magnification range is 40X–400X.

Phase Contrast Optical System

ParameterSpecification
Observation ModePhase Contrast
Optical SystemInfinity Plan
Phase ObjectivesPH10X, PH20X and PH40X
Phase AttachmentCenter-Adjustable Sliding Plate
Phase Annulus Configurations10X and 20X–40X
Centering TelescopeØ30 mm
CondenserSuper Long Working Distance
Condenser N.A.0.3
Condenser Working Distance72 mm
Illumination6V / 30W Halogen
Main PurposeObservation of Transparent and Low-Contrast Biological Specimens

Stage and Culture Vessel Compatibility

ParameterSpecification
Stage TypeLarge Plane Stage
Stage Dimensions160 × 250 mm
Condenser Working Distance72 mm
Clearance Without CondenserUp to 150 mm
Compatible SpecimensSuitable Culture Dishes, Flasks and Laboratory Vessels
Observation ArrangementInverted
Specimen AccessFrom Above the Stage

Compatibility with individual culture vessels depends on the vessel dimensions, bottom thickness and optical properties.

Digital Imaging Compatibility

ParameterSpecification
Viewing Head OptionsBinocular / Trinocular
Camera AttachmentSupported with Trinocular Configuration
Camera ResolutionNot Specified
Image CaptureDepends on Installed Camera
Video RecordingDepends on Installed Camera
Imaging SoftwareNot Specified
Computer ConnectivityDepends on Selected Camera System

Microscope Compatibility and Usage

The KCX-173 Inverted Tissue Culture Microscope is designed for observation of suitable tissue cultures, cell cultures and biological specimens.

It can be used in biotechnology laboratories, microbiology facilities, pharmaceutical research centers, universities and scientific institutions.

For routine observation, the specimen should be placed in a compatible culture vessel on the microscope stage.

The appropriate bright-field or phase-contrast objective should be selected according to the specimen.

For phase-contrast observation, the corresponding phase annulus should be aligned correctly.

The microscope's focusing controls allow adjustment of image sharpness.

The halogen illumination system provides transmitted light for observation.

The optical filters can be used according to the selected illumination requirements.

For extended live-cell observation, additional environmental control equipment may be necessary.

The microscope should be operated according to its final supplied technical configuration and applicable laboratory safety procedures.

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