PHASE CONTRAST MICROSCOPE KCX-200

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• 1. Advanced Phase Contrast Microscope
• The KCX-200 Phase Contrast Microscope is designed for observation of suitable transparent and low-contrast biological specimens.
• Phase contrast microscopy can make certain specimen structures more visible without requiring conventional staining.
• 2. Biological Research Configuration
• The microscope is intended for biological observation, scientific research and laboratory education.
• 3. Plan DIN Achromatic Optical System
• The KCX-200 incorporates Plan DIN Achromatic objectives designed to provide corrected color performance and a relatively flat image field.
• 4. 45 mm DIN Objective Standard
• The supplied reference specifies a 45 mm DIN objective configuration.
• 5. 4X Plan Achromatic Objective
• The 4X objective supports low-power scanning and locating suitable specimen areas.
• 6. 10X Plan Achromatic Objective
• The 10X objective provides general magnification for observing biological specimens.
• 7. 40X Spring-Loaded Objective
• The microscope includes a 40X spring-loaded objective for higher-magnification observation.
• 8. 100X Oil Immersion Objective
• The KCX-200 includes a 100X spring-loaded oil immersion objective for suitable high-magnification microscopy.
• 9. Wide-Field WF10X Eyepieces
• The microscope incorporates WF10X wide-field eyepieces for binocular observation.
• 10. Optional WF16X Eyepieces
• The reference includes an optional WF16X eyepiece configuration.
• 11. 40X–1600X Magnification Capability
• The microscope supports nominal magnifications from 40X to 1600X, depending on the installed eyepiece and selected objective.
• 12. Sliding Binocular Viewing Head
• The KCX-200 incorporates a sliding binocular observation head.
• 13. Ergonomic 45° Head Inclination
• The viewing head is inclined at 45° for laboratory observation.
• 14. 360° Rotatable Head
• The binocular head supports 360° rotation for flexible viewing orientation.
• 15. Adjustable Interpupillary Distance
• The microscope features a 55–75 mm interpupillary adjustment range.
• 16. Double-Layer Mechanical Stage
• The KCX-200 includes a double-layer mechanical stage for specimen positioning.
• 17. 140 × 155 mm Stage
• The microscope features a mechanical stage measuring 140 × 155 mm.
• 18. 70 × 50 mm Stage Movement
• The mechanical stage provides 70 × 50 mm X-Y movement.
• 19. Abbe Condenser
• The microscope incorporates an Abbe condenser for transmitted-light observation.
• 20. Condenser N.A. 1.25
• The condenser has a specified numerical aperture of 1.25.
• 21. Adjustable Iris Diaphragm
• The illumination system includes an iris diaphragm with a specified opening range of Ø2–Ø30 mm.
• 22. Ø32 mm Optical Filter
• The microscope supports a specified Ø32 mm filter.
• 23. Coaxial Coarse Focusing
• The microscope features a coaxial coarse focusing control for initial specimen focusing.
• 24. Coaxial Fine Focusing
• The KCX-200 includes fine focusing for precise adjustment of image sharpness.
• 25. 25 mm Focusing Travel
• The focusing mechanism provides a specified travel range of 25 mm.
• 26. 2 µm Fine Focusing Precision
• The reference specifies fine focusing precision of 0.002 mm, equivalent to 2 micrometers.
• 27. Halogen Illumination Option
• The microscope is available in a referenced 6V / 20W halogen illumination configuration.
• 28. Incandescent Illumination Option
• The supplied reference lists a 20W incandescent illumination option.
• 29. Fluorescent Lamp Option
• The reference includes a 5W fluorescent lamp option for transmitted illumination.
• This should not be confused with a fluorescence microscopy excitation system.
• 30. LED Illumination Option
• The microscope is also listed with a 1W LED illumination option.
• 31. Rechargeable LED Configuration
• An optional rechargeable LED arrangement is referenced.
• 32. Low-Contrast Specimen Observation
• Phase contrast microscopy supports observation of suitable specimens with limited natural contrast.
• 33. Unstained Biological Observation
• A properly configured phase contrast system can support examination of suitable unstained specimens.
• 34. Research and Educational Applications
• The KCX-200 is intended for microscopy training and appropriate biological research applications.
• 35. KC Scientific Works Phase Contrast Microscope
• The KCX-200 Phase Contrast Microscope is offered under the KC SCIENTIFIC WORKS biological and research microscope range.

The Phase Contrast Microscope KCX-200 is intended for examining transparent and low-contrast biological specimens.

Many biological specimens contain structures that are difficult to distinguish using conventional bright-field illumination because they absorb very little light.

Phase contrast microscopy uses differences in optical path length to create visible image contrast.

This technique can help researchers observe suitable unstained cells, microorganisms and other transparent biological specimens.

The KCX-200 reference specifies Plan DIN Achromatic objectives, a sliding binocular viewing head, a double-layer mechanical stage, an Abbe condenser and precision coaxial focusing.

The microscope offers multiple illumination configurations, including halogen, incandescent, fluorescent-lamp and LED options.

Its optical magnification range depends on the installed eyepieces and objective lenses.

The KCX-200 is intended for biological education, cell observation, microbiology, tissue culture research and appropriate laboratory microscopy.

Important: Functional phase contrast observation requires compatible phase objectives and matched phase annuli or a suitable phase contrast condenser arrangement. These components are not detailed in the supplied reference and must be confirmed for the final KCX-200 configuration.

Applications

1. Cell Biology

The KCX-200 Phase Contrast Microscope can support examination of suitable transparent cell specimens.

2. Microbiology

A correctly configured phase contrast system can assist in observation of appropriate microorganisms.

3. Biotechnology Laboratories

The microscope is suitable for relevant biological microscopy applications in biotechnology research.

4. Tissue Culture Laboratories

Phase contrast microscopy can support examination of suitable cell cultures and unstained specimens.

5. Biological Research

The KCX-200 can be used for observation of compatible biological samples.

6. Pharmaceutical Research

The microscope can support appropriate specimen observation in pharmaceutical research environments.

7. Medical Research

Research laboratories can use phase contrast microscopy for suitable biological investigations.

8. Clinical Laboratory Research

The microscope can support research and educational microscopy involving prepared biological samples.

9. Universities

The KCX-200 is suitable for advanced biological microscopy education.

10. Medical Colleges

The microscope can support teaching of phase contrast microscopy principles and specimen observation.

11. Research Institutions

The KCX-200 can be used for suitable microscopy research applications.

12. Life Science Laboratories

The microscope supports observation of transparent and low-contrast biological specimens.

13. Educational Laboratories

Students can learn optical microscopy, specimen preparation and focusing techniques.

14. Environmental Microbiology

Phase contrast microscopy can support examination of suitable environmental microorganism samples.

15. Water Microbiology

The microscope can be used for observation of appropriate microorganisms in prepared water samples.

16. Pharmaceutical Laboratories

The KCX-200 can support suitable microscopy-based laboratory research and training.

17. Quality-Control Laboratories

The microscope can be used for applicable optical inspection and specimen observation.

18. Unstained Cell Observation

Phase contrast microscopy supports visualization of suitable cells without routine staining.

19. Microorganism Observation

The microscope can assist in examining compatible transparent microorganisms.

20. Scientific Microscopy Training

The KCX-200 supports practical training in phase contrast and transmitted-light microscopy.

Typical Specimens and Observations

Phase Contrast Observation System

Phase contrast microscopy is an optical technique used to convert differences in the phase of transmitted light into visible differences in image intensity.

It is particularly useful for transparent specimens that show limited contrast under ordinary bright-field illumination.

A complete phase contrast system normally includes compatible phase contrast objectives and corresponding condenser annuli.

The optical configuration must be properly matched and aligned for effective phase contrast observation.

Phase Contrast System Details

Optical System and Objectives

The listed objective specifications describe the reference optical configuration. Their phase contrast compatibility must be verified separately.

Magnification System

Total optical magnification is calculated using:

Total Magnification = Eyepiece Magnification × Objective Magnification

Standard WF10X Configuration

Optional WF16X Configuration

Maximum stated nominal magnification: 1600X with the optional WF16X eyepiece and 100X objective.

Mechanical Stage

The KCX-200 features a double-layer mechanical stage for controlled positioning of microscope slides.

Stage Specifications

Condenser and Diaphragm System

The microscope includes an Abbe condenser with numerical aperture 1.25.

The condenser supports transmitted-light illumination by directing light toward the specimen.

The iris diaphragm allows adjustment of the illumination aperture.

Condenser Specifications

Focusing System

The KCX-200 Phase Contrast Microscope incorporates coaxial coarse and fine focusing controls.

The coarse focusing mechanism supports initial specimen focusing.

The fine focusing mechanism allows smaller adjustments for image clarity.

Focusing Specifications

Illumination Configurations

The reference specifies several alternative illumination options.

These are alternative configurations, not necessarily lamps supplied together with one microscope.

Working Principle

The Phase Contrast Microscope KCX-200 is intended to operate using the principle of phase contrast microscopy.

Transparent biological specimens can alter the phase of transmitted light without absorbing much of it.

Under ordinary bright-field illumination, these phase differences may be difficult to observe.

A properly configured phase contrast microscope uses a condenser annulus and a corresponding phase plate in the objective.

The illumination system produces a hollow cone of light directed toward the specimen.

Light passing through the specimen is affected by differences in refractive index and thickness.

The phase contrast optical system causes appropriate interference between direct and diffracted light.

This converts optical phase differences into visible intensity differences.

As a result, suitable transparent structures become easier to distinguish.

The microscope's mechanical stage allows controlled positioning of the specimen.

The coaxial focusing system is used to obtain a clear image.

Basic Phase Contrast Observation Process

1. Specimen Preparation: Prepare a suitable transparent or low-contrast specimen.

2. Slide Positioning: Place the specimen on the mechanical stage.

3. Illumination: Switch on the selected transmitted-light illumination system.

4. Objective Selection: Select the required compatible objective.

5. Initial Focusing: Locate the specimen using coarse and fine focusing controls.

6. Phase Component Selection: Select the matching phase condenser annulus where fitted.

7. Phase Alignment: Align the phase optical system using the appropriate supplied method.

8. Observation: Examine the specimen through the binocular eyepieces.

9. Stage Movement: Move the specimen using the mechanical stage controls.

10. Magnification Adjustment: Change objectives as required, using the corresponding compatible phase components.

Educational and Research Benefits

The KCX-200 Phase Contrast Microscope supports training and research involving optical microscopy.

It helps users study:

• Phase contrast microscopy principles

• Transparent specimen observation

• Unstained biological specimens

• Cell biology

• Microbiology

• Tissue culture observation

• Optical magnification

• Objective selection

• Condenser adjustment

• Coaxial focusing

• Mechanical stage operation

• Biological specimen preparation

• Bright-field and phase contrast differences

• Laboratory microscopy techniques

Microscope Compatibility and Usage

The KCX-200 Phase Contrast Microscope is intended for appropriate biological and laboratory microscopy applications.

It can be used in research institutions, universities, biotechnology laboratories, microbiology departments and educational facilities.

The microscope should be placed on a stable laboratory surface.

Specimens should be prepared using appropriate laboratory procedures.

The illumination configuration should be selected according to the supplied model.

Observation should begin with a lower-power objective.

Coarse and fine focusing controls should be adjusted carefully.

For phase contrast microscopy, the installed phase objectives and condenser annuli must be correctly matched.

The optional WF16X eyepieces can be used where compatible with the supplied optical configuration.

The 100X oil immersion objective should be used with suitable immersion oil.

After use, the optical components should be cleaned with appropriate optical cleaning materials.

The microscope should be stored in a clean, dry environment.