TISSUE CULTURE MICROSCOPE KCX-168

• 1. Advanced Tissue Culture Microscope
• The KCX-168 Tissue Culture Microscope is designed for observation of cultured cells and suitable biological specimens.
• 2. Inverted Optical Design
• The microscope uses an inverted optical arrangement, positioning the objective system below the specimen stage.
• 3. Infinity-Corrected Optical System
• The reference specifies infinity-corrected optics for use with compatible objectives and optical components.
• 4. Trinocular Observation Head
• The microscope incorporates a trinocular head for visual observation and camera-based imaging.
• 5. Selectable Light Distribution
• The reference specifies 100:0 and 0:100 light distribution settings.
• 6. 10X Wide-Field Eyepieces
• The KCX-168 reference configuration includes 10X eyepieces.
• 7. 23 mm Field Number
• The eyepieces feature a specified field number of 23 mm.
• 8. Diopter Adjustment
• The viewing system includes diopter adjustment for individual optical correction.
• 9. Anti-Fungus Eyepiece Treatment
• The reference identifies anti-fungus treatment for the eyepieces.
• 10. Brightfield Observation
• The microscope supports conventional transmitted-light brightfield microscopy.
• 11. Phase Contrast Observation
• The phase contrast system supports examination of suitable low-contrast biological specimens.
• 12. Fluorescence Observation
• The epi-fluorescence system supports imaging of specimens prepared with compatible fluorescent labels.
• 13. Optional DIC Observation
• Differential interference contrast is identified as an upgrade option.
• 14. Optional Emboss Contrast
• The reference identifies emboss contrast, or pseudo-3D contrast, as an optional observation method.
• 15. Focusable ELWD Condenser
• The microscope incorporates a focusable extra-long-working-distance condenser.
• 16. Centerable Phase Slider
• The condenser system includes a centerable phase contrast slider.
• 17. Encoded Quadruple Nosepiece
• The KCX-168 reference configuration features a four-position encoded nosepiece.
• 18. DIC Upgrade Slot
• The nosepiece arrangement includes a slot intended for compatible DIC components.
• 19. Plan Achromat Objectives
• The microscope supports compatible plan achromatic long-working-distance objectives.
• 20. Plan Fluor Objectives
• The reference also specifies plan fluor long-working-distance objectives.
• 21. Long-Working-Distance Optical Design
• The objective system is intended for observation through compatible culture vessel bottoms.
• 22. Optional 100X Oil Immersion Objective
• The reference identifies an upgrade option up to 100X oil immersion, subject to optical compatibility.
• 23. Attachable Mechanical Stage
• The microscope incorporates an attachable mechanical stage for specimen positioning.
• 24. Universal Specimen Holder
• The stage system includes a universal holder for suitable laboratory vessels.
• 25. Culture Vessel Observation
• The inverted design supports observation of compatible culture dishes and flasks.
• 26. Cool White LED Illumination
• The reference specifies a cool white transmitted-light LED source.
• 27. 20–30 W LED Power
• The transmitted illumination system has a stated power range of 20–30 W.
• 28. Long-Life Transmitted Illumination
• The reference claims an LED operational lifespan exceeding 60,000 hours.
• 29. Epi-Fluorescence Attachment
• The microscope incorporates an epi-fluorescence observation system.
• 30. Three Fluorescence Filter Channels
• The reference supports up to three fluorescence filter cubes.
• 31. DAPI Fluorescence Filter
• The reference includes a DAPI-compatible fluorescence filter set.
• 32. FITC Fluorescence Filter
• The microscope configuration includes a FITC-compatible filter set.
• 33. TRITC Fluorescence Filter
• The reference includes a TRITC-compatible fluorescence filter set.
• 34. Scientific CMOS Camera
• The KCX-168 reference configuration specifies a 5.9 MP scientific CMOS camera.
• 35. USB 3.0 Imaging Interface
• The camera uses a USB 3.0 interface for connection to compatible computer equipment.
• 36. Fluorescence Image Analysis
• The reference software supports fluorescence channel processing and image analysis.
• 37. Time-Lapse Imaging
• The specified software includes time-lapse imaging functionality.
• 38. Cell Counting Tools
• The software reference includes automatic and manual cell counting.
• 39. Measurement and Annotation
• The imaging software supports measurements, image annotations and analysis tools.
• 40. KC Scientific Works Tissue Culture Microscope
• The KCX-168 Tissue Culture Microscope is presented under the KC SCIENTIFIC WORKS inverted and advanced biological microscopy range.

The Tissue Culture Microscope KCX-168 is intended for observation and imaging of cultured cells, biological tissues and suitable laboratory specimens.

Unlike conventional upright microscopes, the inverted optical system positions the objectives below the specimen stage.

This arrangement is useful for examining cells located near the bottom of compatible culture vessels.

Brightfield microscopy provides transmitted-light observation of suitable specimens.

Phase contrast microscopy improves the visibility of low-contrast structures without requiring fluorescence labeling.

Fluorescence microscopy allows observation of compatible fluorescent labels using suitable excitation and emission filters.

The trinocular observation head supports visual examination and digital imaging.

The mechanical stage allows specimen positioning.

The scientific CMOS camera supports digital image acquisition according to the camera's verified performance.

The imaging software provides tools for fluorescence analysis, measurement, annotation and cell counting.

The KCX-168 is intended for cell culture research, biotechnology, pharmaceutical research, academic laboratories and suitable biological imaging workflows.

Applications

1. Tissue Culture Laboratories

The KCX-168 Tissue Culture Microscope supports observation of suitable cultured cell specimens.

2. Live Cell Imaging

The microscope can be used for imaging living cells under appropriate laboratory conditions.

3. Cell Biology Research

The KCX-168 supports examination of cellular morphology and biological structures.

4. Biotechnology Laboratories

The microscope supports suitable biotechnology research and imaging activities.

5. Pharmaceutical Research

The KCX-168 can be used for cell-based research observation.

6. Fluorescence Microscopy

The microscope supports imaging of appropriately fluorescently labeled specimens.

7. Phase Contrast Microscopy

The KCX-168 supports observation of suitable low-contrast cell specimens.

8. Brightfield Microscopy

The microscope can be used for conventional transmitted-light specimen observation.

9. Cell Culture Monitoring

The KCX-168 supports visual monitoring of suitable cell cultures.

10. Cell Morphology Studies

The microscope can be used to examine cellular shape and structural changes.

11. Fluorescence Channel Imaging

The KCX-168 supports imaging using compatible DAPI, FITC and TRITC filter sets.

12. Time-Lapse Research

The reference software supports repeated image capture for suitable time-lapse experiments.

13. Cell Counting

The microscope's specified software includes automatic and manual cell counting tools.

14. Tissue Research

The KCX-168 supports observation of suitable biological tissue specimens.

15. Academic Research Laboratories

The microscope can be used for advanced biological microscopy education and research.

16. Molecular Biology Research

The KCX-168 supports observation of suitably labeled biological specimens.

17. Immunofluorescence Studies

The microscope can be used with appropriate fluorescence-labeled samples.

18. Biomedical Research

The KCX-168 supports suitable non-diagnostic biomedical imaging activities.

19. Cell-Based Assays

The microscope can be used for visual analysis in suitable cell-based research workflows.

20. Scientific Image Documentation

The camera and imaging software support digital recording and analysis of microscope images.

Typical Specimens and Observations

Working Principle

The Tissue Culture Microscope KCX-168 operates on the principle of inverted optical microscopy with brightfield, phase contrast and fluorescence observation.

The objective lenses are positioned below the specimen stage.

Suitable specimens are placed in compatible culture vessels or specimen holders.

For brightfield observation, transmitted light passes through the specimen and is collected by the objective.

For phase contrast observation, compatible phase optics convert phase differences in the specimen into observable intensity variations.

For fluorescence observation, excitation light passes through the appropriate optical filter arrangement and reaches the labeled specimen.

Fluorescent molecules emit light at wavelengths determined by their optical properties.

The fluorescence filter system helps separate the desired emission from excitation light.

The objective collects the specimen image, which is directed toward the binocular viewing system or camera path.

The scientific CMOS camera converts the optical image into digital image data.

The software processes the images for visualization, measurement and analysis.

Basic Observation Procedure

1. Microscope Setup: Place the KCX-168 on a stable laboratory surface.

2. System Inspection: Check the microscope, stage, objectives and imaging connections.

3. Illumination Setup: Activate the appropriate illumination system.

4. Specimen Selection: Choose a suitable prepared specimen or culture vessel.

5. Specimen Placement: Position the specimen using the appropriate holder.

6. Observation Mode: Select brightfield, phase contrast or fluorescence.

7. Objective Selection: Begin with an appropriate lower-power objective.

8. Initial Focusing: Bring the specimen into focus using the microscope's focusing system.

9. Eyepiece Adjustment: Adjust the viewing system for comfortable observation.

10. Stage Positioning: Move the mechanical stage to locate the desired specimen area.

11. Image Optimization: Adjust illumination and contrast according to the selected mode.

12. Fluorescence Filter Selection: Select the appropriate filter set for fluorescent specimens.

13. Camera Activation: Connect the scientific CMOS camera to the imaging computer.

14. Software Setup: Open the compatible imaging software.

15. Image Capture: Acquire images using suitable exposure settings.

16. Image Analysis: Apply the required measurement or fluorescence analysis tools.

17. Data Recording: Save the acquired images and associated observations.

18. Completion: Turn off illumination and secure the microscope after use.

Fluorescence illumination and live-cell handling should follow the laboratory's approved safety and specimen-care procedures.

Magnification System

The KCX-168 reference specifies 10X eyepieces and Plan Achromat / Plan Fluor long-working-distance objectives.

However, the individual objective magnifications are not provided.

Total Visual Magnification = Eyepiece Magnification × Objective Magnification

For example, a compatible 20X objective combined with a 10X eyepiece would produce 200X nominal visual magnification.

This is a mathematical example, not confirmation that a 20X objective is supplied with KCX-168.

Infinity-Corrected Optical System

The microscope incorporates an infinity-corrected optical design.

Optical Specifications

Trinocular Viewing System

The KCX-168 features a trinocular observation head.

Viewing Specifications

Long-Working-Distance Objective System

The microscope reference includes Plan Achromat and Plan Fluor LWD objectives.

Objective Specifications

ELWD Condenser and Phase Contrast

The KCX-168 reference specifies a focusable ELWD condenser with a centerable phase slider.

Condenser Specifications

Verification note: An ELWD condenser with N.A. 1.25 is an unusual combination. The numerical aperture and working-distance specification should be checked against the final condenser model.

Mechanical Stage and Specimen Holder

The microscope incorporates an attachable mechanical stage.

Stage Specifications

LED Transmitted Illumination

The KCX-168 reference specifies a cool white LED illumination system.

Illumination Specifications

Epi-Fluorescence Imaging System

The KCX-168 incorporates a reference epi-fluorescence attachment with a rotating filter turret.

Fluorescence Specifications

DAPI Channel

The DAPI filter set is intended for observation of specimens labeled with compatible blue-emitting fluorescent markers.

FITC Channel

The FITC filter set supports imaging of specimens labeled with compatible green-emitting fluorophores.

TRITC Channel

The TRITC filter set supports observation of compatible orange-red fluorescent labels.

Actual excitation and emission wavelengths depend on the installed filter cubes and should be confirmed separately.

Scientific CMOS Camera

The KCX-168 reference configuration includes a 5.9 MP scientific CMOS camera.

Camera Specifications

The 10–15 fps value is not associated with a specified resolution or acquisition mode in the reference. Model-specific camera documentation should establish the verified frame rate.

Advanced Imaging and Fluorescence Analysis Software

The reference software includes digital imaging and fluorescence analysis functions.

Software Features

These functions should be verified against the final supplied software version and license.

Computer System Configuration

The supplied reference includes the following computer configuration.

Computer inclusion, exact hardware model and operating-system compatibility should be confirmed in the final supply scope.

Educational and Research Benefits

The KCX-168 Tissue Culture Microscope supports:

• Tissue culture observation

• Live cell imaging

• Cell morphology examination

• Brightfield microscopy

• Phase contrast microscopy

• Fluorescence microscopy

• DAPI fluorescence observation

• FITC fluorescence observation

• TRITC fluorescence observation

• Digital image acquisition

• Scientific image documentation

• Cell counting

• Fluorescence intensity analysis

• Image measurement

• Time-lapse imaging

• Cell-based research

• Biotechnology studies

• Pharmaceutical research

• Academic microscopy training

• Biological image analysis

Microscope Compatibility and Usage

The KCX-168 Tissue Culture Microscope is intended for research laboratories, biotechnology facilities, cell biology laboratories and academic institutions.

Suitable specimens should be placed in compatible culture vessels.

The universal specimen holder should be selected according to the vessel dimensions.

The appropriate objective should be selected for the required observation mode.

Brightfield illumination can be used for conventional transmitted-light observation.

Phase contrast can be used for suitable low-contrast specimens.

Fluorescence observation requires appropriate fluorescent labeling and compatible filter sets.

The scientific CMOS camera can be used for digital image capture according to its verified specifications.

The imaging software can be used for measurement, annotation and fluorescence image processing.

For live-cell experiments, environmental control requirements such as temperature, humidity and gas composition must be addressed separately. An environmental incubation chamber is not specified in the supplied reference.